Purified Rabbit anti-FOXO1 Antibody

SKU: DL94466A

Size 1: 100 ul

Synonyms : FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to FOXO1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO1. The exact sequence is proprietary.

WB description: Western blot analysis of FOXO1 expression in HEK293T (A), Hela (B), rat brain (C), mouse brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of FOXO1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of FOXO1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)

Gene ID(Human): 2308

Gene ID (Mouse): 56458

Gene ID(Rat): 84482

SwissProt (Human): Q12778

SwissProt (Mouse): Q9R1E0

SwissProt (Rat): G3V7R4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.