Purified Rabbit anti-ETFA Antibody

SKU: DL96491A

Size 1: 100 ul

Synonyms : Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to ETFA

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human ETFA

WB description: Western blot analysis of ETFA expression in A549 (A), HepG2 (B), mouse kidney (C), mouse heart (D), rat liver (E) whole cell lysates.

IHC description: Immunohistochemical analysis of ETFA staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ETFA staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 2108

Gene ID (Mouse): 110842

Gene ID(Rat): 300726

SwissProt (Human): P13804

SwissProt (Mouse): Q99LC5

SwissProt (Rat): P13803

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.