Purified Rabbit anti-EEF2 (pT56) Antibody

SKU: DL92908A

Size 1: 100 ul

Synonyms : EF2; Elongation factor 2; EF-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Mk, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to EEF2 (pT56)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EEF2 (pT56). The exact sequence is proprietary.

WB description: Western blot analysis of EEF2 (pT56) expression in SKOV3 (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of EEF2 (pT56) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of EEF2 (pT56) staining in SKOV3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 1938

Gene ID (Mouse): 13629

Gene ID(Rat): 29565

SwissProt (Human): P13639

SwissProt (Mouse): P58252

SwissProt (Rat): P05197

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.