Purified Rabbit anti-Dopamine Receptor D3 Antibody

SKU: DL97601A

Size 1: 100 ul

Synonyms : D(3) dopamine receptor; Dopamine D3 receptor

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Dopamine Receptor D3

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Dopamine Receptor D3

WB description: Western blot analysis of Dopamine Receptor D3 expression in mouse brain (A) whole cell lysates.

IHC description: Immunohistochemical analysis of Dopamine Receptor D3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Dopamine Receptor D3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 1814

Gene ID (Mouse): 13490

Gene ID(Rat): 29238

SwissProt (Human): P35462

SwissProt (Mouse): P30728

SwissProt (Rat): P19020

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.