Purified Rabbit anti-Cytochrome b5A Antibody

SKU: DL90305A

Size 1: 100 ul

Synonyms : CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Cytochrome b5A

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome b5A. The exact sequence is proprietary.

WB description: Western blot analysis of Cytochrome b5A expression in HepG2 (A), HeLa (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Cytochrome b5A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Cytochrome b5A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 1528

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P00167

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.