Purified Rabbit anti-Cortactin (pY421) Antibody

SKU: DL92212A

Size 1: 100 ul

Synonyms : EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to Cortactin (pY421)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cortactin (pY421). The exact sequence is proprietary.

WB description: Western blot analysis of Cortactin (pY421) expression in HeLa UV-treated (A), NIH3T3 H2O2-treated (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Cortactin (pY421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 2017

Gene ID (Mouse): 13043

Gene ID(Rat):

SwissProt (Human): Q14247

SwissProt (Mouse): Q60598

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.