Purified Rabbit anti-CK1 gamma 1 Antibody

SKU: DL92415A

Size 1: 100 ul

Synonyms : Casein kinase I isoform gamma-1; CKI-gamma 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to CK1 gamma 1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CK1 gamma 1. The exact sequence is proprietary.

WB description: Western blot analysis of CK1 gamma 1 expression in Jurkat (A), NIH3T3 (B), H9C2 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of CK1 gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of CK1 gamma 1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 53944

Gene ID (Mouse): 214897

Gene ID(Rat): 64086

SwissProt (Human): Q9HCP0

SwissProt (Mouse): Q8BTH8

SwissProt (Rat): Q62761

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.