Purified Rabbit anti-CDK8 Antibody

SKU: DL92171A

Size 1: 100 ul

Synonyms : Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Z

Applications: WB, IHC

Background: Rabbit polyclonal antibody to CDK8

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK8. The exact sequence is proprietary.

WB description: Western blot analysis of CDK8 expression in HepG2 (A), A549 (B), Raw264.7 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 1024

Gene ID (Mouse): 264064

Gene ID(Rat):

SwissProt (Human): P49336

SwissProt (Mouse): Q8R3L8

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.