Purified Rabbit anti-CaMK2 alpha/delta (pT286) Antibody

SKU: DL92812A

Size 1: 100 ul

Synonyms : CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Mk, P, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to CaMK2 alpha/delta (pT286)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CaMK2 alpha/delta (pT286). The exact sequence is proprietary.

WB description: Western blot analysis of CaMK2 alpha/delta (pT286) expression in HeLa (A) whole cell lysates.

IHC description: Immunohistochemical analysis of CaMK2 alpha/delta (pT286) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of CaMK2 alpha/delta (pT286) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 815; 817

Gene ID (Mouse): 12322; 108058

Gene ID(Rat): 25400; 24246

SwissProt (Human): Q9UQM7; Q13557

SwissProt (Mouse): P11798; Q6PHZ2

SwissProt (Rat): P11275; P15791

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.