Purified Rabbit anti-c-Jun (pS73/100) Antibody

SKU: DL93698A

Size 1: 100 ul

Synonyms : JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUND; Transcription factor jun-D

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, P, Z

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to c-Jun (pS73/100)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-Jun. The exact sequence is proprietary.

WB description: Western blot analysis of MEF2A (pT312) expression in HeLa (A), mouse liver (B), NIH3T3 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of MEF2A (pT312) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of MEF2A (pT312) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 4205

Gene ID (Mouse): 17258

Gene ID(Rat): 309957

SwissProt (Human): Q02078

SwissProt (Mouse): Q60929

SwissProt (Rat): Q2MJT0

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.