Purified Rabbit anti-Brn-5 Antibody

SKU: DL96427A

Size 1: 100 ul

Synonyms : BRN5; MPOU; TCFB1; POU domain, class 6, transcription factor 1; Brain-specific homeobox/POU domain protein 5; Brain-5; Brn-5; mPOU homeobox protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to Brn-5

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Brn-5

WB description: Western blot analysis of Brn-5 expression in HepG2 (A), Raji (B), mouse liver (C), rat kidney (D) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of Brn-5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)

Gene ID(Human): 5463

Gene ID (Mouse): 19009

Gene ID(Rat): 116545

SwissProt (Human): Q14863

SwissProt (Mouse): Q07916

SwissProt (Rat): P56223

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.