Purified Rabbit anti-Beclin-1 Antibody

SKU: DL96714A

Size 1: 100 ul

Synonyms : GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to Beclin-1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Beclin-1

WB description: Western blot analysis of Beclin-1 expression in A431 (A), MCF7 (B), Raji (C), mouse kidney (D), rat brain (E) whole cell lysates.

IHC description: Immunohistochemical analysis of Beclin-1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)

Gene ID(Human): 8678

Gene ID (Mouse): 56208

Gene ID(Rat): 114558

SwissProt (Human): Q14457

SwissProt (Mouse): O88597

SwissProt (Rat): Q91XJ1

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.