Purified Rabbit anti-BAF57 Antibody

SKU: DL97106A

Size 1: 100 ul

Synonyms : BAF57; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BRG1-associated factor 57; BAF57

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP, ChIP

Background: Rabbit polyclonal antibody to BAF57

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human BAF57

WB description: Western blot analysis of BAF57 expression in K562 (A), SHSY5Y (B), SKOV3 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of BAF57 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of BAF57 staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/50 - 1/200), ChIP (1/20 - 1/100)

Gene ID(Human): 6605

Gene ID (Mouse): 57376

Gene ID(Rat): 303518

SwissProt (Human): Q969G3

SwissProt (Mouse): O54941

SwissProt (Rat): Q56A18

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.