Purified Rabbit anti-Alpha-adducin (pS726/713) Antibody

SKU: DL92470A

Size 1: 100 ul

Synonyms : ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha; ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, P

Applications: WB, IF/IC, IP

Background: Rabbit polyclonal antibody to Alpha-adducin (pS726/713)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-adducin (pS726/713). The exact sequence is proprietary.

WB description: Western blot analysis of Alpha-adducin (pS726/713) expression in HeLa (A), SP2/0 (B), PC12 (C) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of Alpha-adducin (pS726/713) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 118; 119

Gene ID (Mouse): 11518; 11519

Gene ID(Rat): 24170; 24171

SwissProt (Human): P35611; P35612

SwissProt (Mouse): Q9QYC0; Q9QYB8

SwissProt (Rat): Q63028; Q05764

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.