Purified Rabbit anti-ADAR2 Antibody

SKU: DL90017A

Size 1: 100 ul

Synonyms : ADAR2; DRADA2; RED1; Double-stranded RNA-specific editase 1; RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to ADAR2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADAR2. The exact sequence is proprietary.

WB description: Western blot analysis of ADAR2 expression in HepG2 (A), MCF7 (B), mouse brain (C), rat brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of ADAR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ADAR2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 104

Gene ID (Mouse): 110532

Gene ID(Rat): 25367

SwissProt (Human): P78563

SwissProt (Mouse): Q91ZS8

SwissProt (Rat): P51400

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.