Purified Rabbit anti-ACTN1/2/3/4 Antibody

SKU: DL92466A

Size 1: 100 ul

Synonyms : ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN4; Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Z

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to ACTN1/2/3/4

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN1/2/3/4. The exact sequence is proprietary.

WB description: Western blot analysis of ACTN1/2/3/4 expression in HeLa (A), HuvEc (B), mouse muscle (C), PC12 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 87; 88; 89; 81

Gene ID (Mouse): 109711; 11472; 11474; 60595

Gene ID(Rat): 81634; 63836

SwissProt (Human): P12814; P35609; Q08043; O43707

SwissProt (Mouse): Q7TPR4; Q9JI91; O88990; P57780

SwissProt (Rat): Q9Z1P2; Q9QXQ0

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.