Purified Rabbit anti-4EBP1 Antibody

SKU: DL92915A

Size 1: 100 ul

Synonyms : Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to 4EBP1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 4EBP1. The exact sequence is proprietary.

WB description: Western blot analysis of 4EBP1 expression in HEK293T (A), HepG2 (B), mouse brain (C), rat liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of 4EBP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of 4EBP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 1978

Gene ID (Mouse): 13685

Gene ID(Rat): 116636

SwissProt (Human): Q13541

SwissProt (Mouse): Q60876

SwissProt (Rat): Q62622

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.