Purified Rabbit anti-14-3-3 zeta (pT232) Antibody

SKU: DL94107A

Size 1: 100 ul

Synonyms : 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, P, S

Applications: WB, IHC

Background: Rabbit polyclonal antibody to 14-3-3 zeta (pT232)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 14-3-3 zeta. The exact sequence is proprietary.

WB description: Western blot analysis of 14-3-3 zeta (pT232) expression in A431 UV-treated (A), K562 UV-treated (B), NIH3T3 UV-treated (C) whole cell lysates.

IHC description: Immunohistochemical analysis of 14-3-3 zeta (pT232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 7534

Gene ID (Mouse): 22631

Gene ID(Rat): 25578

SwissProt (Human): P63104

SwissProt (Mouse): P63101

SwissProt (Rat): P63102

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.