Purified Rabbit anti-14-3-3 zeta Antibody

SKU: DL91244A

Size 1: 100 ul

Synonyms : 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, P, S, Z

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to 14-3-3 zeta

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.

WB description: Western blot analysis of Cyclin A1/2 expression in HEK293T (A), NIH3T3 (B), rat brain (C), SW626 (D), SKOV3 (E), A2780 (F) whole cell lysates.

IHC description: Immunohistochemical analysis of Cyclin A1/2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Cyclin A1/2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), ChIP (1/100 - 1/500)

Gene ID(Human): 8900; 890

Gene ID (Mouse): 12427; 12428

Gene ID(Rat): 295052

SwissProt (Human): P78396; P20248

SwissProt (Mouse): Q61456; P51943

SwissProt (Rat): Q6AY13

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.