Purified Rabbit anti-14-3-3 beta Antibody

SKU: DL97188A

Size 1: 100 ul

Synonyms : 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to 14-3-3 beta

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human 14-3-3 beta

WB description: Western blot analysis of 14-3-3 beta expression in SW480 (A), HepG2 (B), mouse liver (C), mouse kidney (D) whole cell lysates.

IHC description: Immunohistochemical analysis of 14-3-3 beta staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of 14-3-3 beta staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 7529

Gene ID (Mouse): 54401

Gene ID(Rat): 56011

SwissProt (Human): P31946

SwissProt (Mouse): Q9CQV8

SwissProt (Rat): P35213

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.