Purified Mouse anti-Actin Muscle Specific Antibody

SKU: DL99514A

Size 1: 100 ul

Synonyms : ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin; ACTA; Actin alpha skeletal muscle; Alpha-actin-1; ACTA3; ACTL3; ACTSG; Actin gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin

host: Mouse

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: IH

Background: Mouse monoclonal antibody to Actin Muscle Specific

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Actin Muscle Specific. The exact sequence is proprietary.

WB description:

IHC description: Immunohistochemical analysis of Actin Muscle Specific staining in human cardiac muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunohistochemical analysis of Actin Muscle Specific staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description: Immunohistochemical analysis of Actin Muscle Specific staining in human fibroid formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

FC description: Immunohistochemical analysis of Actin Muscle Specific staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Conjugation:

Buffer: Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution: IH (1/100 - 1/300)

Gene ID(Human): 70; 58; 72

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P68032; P68133; P63267

SwissProt (Mouse):

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.