Purified Anti-Human HLA-G Antibody[MEM-G/11]

SKU: AN006210P

Size:

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:HLA-G

Target Synonym:HLA;MHC-G;HLA-G;HLA-G HLA-6.0 HLAG;HLA-G5;HLA-6.0;HLAG;alpha chain G;B2 microglobulin;class I;DADB-15K14.8;HLA 6.0;HLA class I histocompatibility antigen;HLA class I histocompatibility antigen alpha chain G;HLA class I molecule;HLA G;HLA G antigen;HLA G histocompatibility antigen class I G;HLA G3;HLA60;HLA-G histocompatibility antigen;Major histocompatibility complex class I G;MHC class I antigen;MHC class I antigen G;MHC G;TCA;T-cell A locus

Research Areas:Immunology

Conjugation:Unconjugated

Host:Mouse

Species reactivity:Human

Application:FCM

Usage:

Isotype:Mouse IgG1, κ

Clonality:Monoclonal

Clone NO.:MEM-G/11

UNIProt ID:P17693

Background:HLA-G Antibody (4H84) is an IgG1 κ mouse monoclonal HLA-G antibody (also designated HLA major histocompatibility complex, class I, G antibody, human leukocyte antigen (HLA) G antibody, 45 kDa transmembrane class I human leukocyte antigen G antibody, chromosome 6p22 gene complex class 1G antibody, placenta trophoblast marker antibody, or fetal derived placental cell marker antibody) that detects the HLA-G protein of mouse, rat and human origin by WB, IP, IF and IHC(P). HLA-G Antibody (4H84) is available as both the non-conjugated anti-HLA-G antibody form, as well as multiple conjugated forms of anti-HLA-G antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Major histocompatibility complex (MHC), human leukocyte antigen (HLA) molecules are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an α or heavy chain, and a non-covalently associated protein, β-2-microglobulin. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Concentration: ≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Human HLA-G protein

Buffer:Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:3135