PRKAG2 Antibody - Cat. #: CSB-PA147130

SKU: CSB-PA147130

Size :100ul

Clone Number:

Aliases:PRKAG25'-AMP-activated protein kinase subunit gamma-2 antibody; AMPK gamma2 antibody; AMPK subunit gamma-2 antibody; H91620p antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:Q9UGJ0

Immunogen:Synthesized peptide derived from internal of Human PRKAG2.

Raised in:Rabbit

Species Reactivity:Human, Mouse

Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

Background:AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Cheung P.C.F., Biochem. J. 346:659-669(2000).
Lang T.M., Genomics 70:258-263(2000).
Ota T., Nat. Genet. 36:40-45(2004).

Clonality:Polyclonal

Isotype:IgG

Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugate:Non-conjugated

Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:PRKAG2

Research Areas:Cardiovascular;Metabolism;Signal transduction