Please see our SYBR Green Brochure
Power Green qPCR Mix
For research use only
Components
|
Component |
P2101-01 |
P2101-02 |
P2101-03 |
|
2X Power Green qPCR Mix |
1 ml |
1 ml × 5 |
1 ml × 25 |
|
|
400 rxn/20μl reaction |
2000 rxn/20μl reaction |
10,000 rxn/20μl reaction |
Storage
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.
Description
Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.
The PowerGreen™ HotStart Taq DNA Polymerase in the mix is chemically modified, and it can reduce non-specific amplification more effectively than the antibody-modified polymerase. At the same time, the mix contains aptamer-based inhibitors, which further reduce the generation of the primer dimer and secondary metabolites. With this dual blocking mechanism, when the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This dual hot-start technique brings greater efficiency and specificity to PCR.
Applications
- Gene expression analysis
- Low-copy gene detection
- Microarray validation
- Gene knockdown validation
Features
- Compatible with all major real-time PCR systems, which accept No ROX (not requiring ROX reference dye), Low ROX or High ROX
- Exceptional specificity with dual hot-start mechanism
- Tight reproducibility in Ct values over a broad dynamic range
- Universal instrument compatibility
Table of Instrument Guide
|
Instruments |
ROX |
Cat. # |
|
ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 |
High ROX |
P2101-01 P2101-02 P2101-03 |
|
ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 |
Low ROX |
P2101-01a P2101-02a P2101-03a |
|
Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler |
No ROX |
P2101-01b P2101-02b P2101-03b |
