PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit

SKU: ELK8250

Size:96T

Reactivity:General

Alternative Names:PtdIns(3,4,5)P3

Assay Type:Competitive Inhibition

Sensitivity:2.84 ng/mL

Standard:500 ng/mL

Detection range:7.82-500 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Signal transduction;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: