Pig Irisin ELISA Kit

SKU: ELK10919

Size:96T

Reactivity:Pig

Alternative Names:FRCP2; Irisin; Fibronectin type III repeat-containing protein 2; FNDC5; Fibronectin Type III Domain Containing Protein 5

Assay Type:Sandwich

Sensitivity:0.12 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:CD & Adhesion molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig Irisin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Irisin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig Irisin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Irisin in the samples is then determined by comparing the OD of the samples to the standard curve.

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