Pig BAFF/CD257(B-Cell Activating Factor) ELISA Kit

SKU: ELK8277

Size:96T

Reactivity:Pig

Alternative Names:CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF

Assay Type:Sandwich

Sensitivity:0.057 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.

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