Pig ANG1(Angiopoietin1) ELISA Kit

SKU: ELK0894

Size:96T

Reactivity:Pig

Alternative Names:ANG1; AGP1; AGPT; ANG-I; ANGPT1; Angiopoietin 1

Assay Type:Competitive Inhibition

Sensitivity:1.15 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:Serum, plasma, other biological fluids

Assay length:2h

Research Area:Tumor immunity;Cardiovascular biology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ANG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ANG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ANG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: