GeneBio Systems
Pig ADST(Androstenone) ELISA Kit
Pig ADST(Androstenone) ELISA Kit
SKU:ELK8586
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Size:96T
Reactivity:Pig
Alternative Names:Androstenone
Assay Type:Competitive Inhibition
Sensitivity:9.68 nmol/mL
Standard:2000 nmol/mL
Detection range:31.25-2000 nmol/mL
Sample type:serum, plasma and other biological fluids
Assay length:2h
Research Area:Biochemical research
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ADST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ADST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ADST in the samples is then determined by comparing the OD of the samples to the standard curve.
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