Phospho-Smad2/3 (Thr8) Antibody , Cat. # AF3367

SKU: AF3367

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:SMAD2,SMAD3

GeneID:4087,4088

Uniprot:P84022/Q15796

Specificity:Phospho-Smad2/3 (Thr8) Antibody detects endogenous levels of Smad2/3 only when phosphorylated at Threonine 8.

Immunogen:A synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Pig,Sheep,Dog,Chicken,Xenopus

Application:WB,IHC,ELISA(peptide)

Dilution:WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2834782

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF3367-BP

Des# Blocking Peptide:Phospho-Smad2/3 (Thr8) Blocking peptide

Blocking Peptide Price:$549 CAD