Phospho-PXN (Tyr31) Antibody - Cat. #: CSB-PA047961

SKU: CSB-PA047961

Size :100ul

Clone Number:

Aliases:FLJ16691 antibody; FLJ23042 antibody; Paired box protein Pax 1 antibody; PAX 1 antibody; PAX1 antibody; PAXI_HUMAN antibody; Paxillin alpha antibody; Paxillin antibody; PXN antibody; PXN protein antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P49023

Immunogen:Peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y(p)-S-Y) derived from Human Paxillin.

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:ELISA, WB; WB:1:500-1:1000

Background:

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Davidson D, et al. (2001) EMBO J 20(13): 3414-3426.
Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96(3): 1123-1128.
Goldberg MB. (2001) Microbiol Mol Biol Rev 65(4): 595-626.
Kook S, et al. (2000) Mol Biol Cell 11(3): 929-939.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:PXN

Research Areas:Cancer;Signal transduction