Phospho-PI3K p85 (Tyr458)[Tyr467]/p55 (Tyr199) Antibody , Cat. # AF3242

SKU: AF3242

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:PIK3R1,PIK3R3

GeneID:5295,8503

Uniprot:P27986/Q92569

Specificity:Phospho-PI3K p85(Tyr458)/p55(Tyr199) Antibody detects endogenous levels of PI3K p85/p55 only when phosphorylated at Tyr467(p85) or Tyr199(p55). The site Tyr467 was historically referenced as Tyr458.

Immunogen:A synthesized peptide derived from human PI3-kinase p85/p55 around the phosphorylation site of Tyr467/199.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat,Monkey

Prediction:Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Application:WB,IHC,IF/ICC,ELISA(peptide)

Dilution:WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2834668

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF3242-BP

Des# Blocking Peptide:Phospho-PI3K p85 (Tyr458)[Tyr467]/p55 (Tyr199) Blocking peptide

Blocking Peptide Price:$549 CAD