Phospho-Occludin (Thr382) Antibody , Cat. # AF4305

SKU: AF4305

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:OCLN

GeneID:100506658

Uniprot:Q16625

Specificity:Phospho-Occludin (Thr382) Antibody detects endogenous levels of Occludin only when phosphorylated at Thr382(mouse).

Immunogen:A synthesized peptide derived from mouse Occludin(Q61146) around the phosphorylation site of Thr382.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Pig,Bovine,Horse

Application:WB,ELISA(peptide)

Dilution:WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2844384

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF4305-BP

Des# Blocking Peptide:Phospho-Occludin (Thr382) Blocking peptide

Blocking Peptide Price:$549 CAD