Phospho-IRS1 (Ser318)[Ser323] Antibody , Cat. # AF4422

SKU: AF4422

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:IRS1

GeneID:3667

Uniprot:P35568

Specificity:Phospho-IRS1 (Ser318) Antibody detects endogenous levels of IRS1 only when phosphorylated at Ser323, which site historically referenced as Ser318.

Immunogen:A synthesized peptide derived from human IRS1 around the phosphorylation site of Ser318.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog

Application:WB,ELISA(peptide)

Dilution:WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2844486

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF4422-BP

Des# Blocking Peptide:Phospho-IRS1 (Ser318)[Ser323] Blocking peptide

Blocking Peptide Price:$549 CAD