Phospho-Histone H2A (Thr121) Antibody , Cat. # AF3135

SKU: AF3135

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:HIST1H2AB; HIST1H2AE,HIST1H2AH,HIST1H2AD,HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM,HIST2H2AA3; HIST2H2AA4,HIST3H2A

GeneID:3012,8335,85235,3013,8329,8330,8332,8336,8969,723790,8337,92815

Uniprot:P0C0S8/P04908/P20671/Q96KK5/Q6FI13/Q7L7L0

Specificity:Phospho-Histone H2A (Thr121) Antibody detects endogenous levels of Histone H2A only when phosphorylated at Threonine 121.

Immunogen:A synthesized peptide derived from human Histone H2A around the phosphorylation site of Thr121.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Bovine,Xenopus

Application:WB,IHC,ELISA(peptide)

Dilution:IHC 1:50-1:200, WB 1:1000, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2834570

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF3135-BP

Des# Blocking Peptide:Phospho-Histone H2A (Thr121) Blocking peptide

Blocking Peptide Price:$549 CAD