Phospho-AMPKBeta1 (S182) Polyclonal Antibody

SKU: BT-PHS00714

Size:100μL

Background:The protein encoded by PRKAB1 (protein kinase AMP-activated non-catalytic subunit beta 1) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Research_area:Signal Transduction; Metabolism

Target_protein:PRKAB1

applications:WB, IHC-p, ELISA

Reactivity:Human, mouse, rat, monkey

Clonality:Polyclonal

Clone ID:

Host:Rabbit

Isotype:IgG

Gene Symbol/ Name:Phospho-AMPKbeta1 (S182) Antibody

Immunogen:The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage:-20°C for 1 year

Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Molecular weight(Da):30382

UniProt accession:Human: Q9Y478; Mouse: Q9R078; Rat: P80386

Synonyms:PRKAB1; AMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb

GeneID:Human: 5564; Mouse: 19079; Rat: 83803