PFN1 ELISA kit (Human)

SKU: SEC233Hu

Size: 96Tests

# of Times Cited in literature: 4

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: PFN1

Target Full Name: Profilin 1

Alternative Names: Epididymis tissue protein Li 184a

Target Species: Human

Uniprot: P07737

Gene ID: 5216

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human PFN1 / Profilin 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 78-5,000pg/mL

Sensitivity: 34pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Profilin 1 (PFN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Profilin 1 (PFN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Profilin 1 (PFN1). No significant cross-reactivity or interference between Profilin 1 (PFN1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Profilin 1 (PFN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Profilin 1 (PFN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Profilin 1 (PFN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Profilin 1 (PFN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Developmental science;

References Citing This Product: Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation

Comparative proteomics analysis of microvesicles in human serum for the evaluation of osteoporosis

Evaluation of profilin 1 as a biomarker in myocardial infarction

Reduced perforin release from T cells as a mechanism underlying hypothermia‐mediated neuroprotection

ЦИркУлИрУЮщИе актИН-СвЯЗЫваЮщИе БелкИ ПрИ ПроГреССИроваНИИ рака ГортаНИ И ГортаНоГлоткИ

The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer

Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status