GeneBio Systems

PCT ELISA kit (Human)

Name: PCT ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEA689Hu
Price: 1176.00 CAD
Availability: InStock

SKU:SEA689Hu

$1,176.00 CAD

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Quantity

Size: 96Tests

# of Times Cited in literature: 16

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: PCT

Target Full Name: Procalcitonin

Alternative Names: Pro-Calcitonin

Target Species: Human

Uniprot: P01258

Gene ID: 796

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human PCT / Procalcitonin

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 31.2-2,000pg/mL

Sensitivity: 12.4pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procalcitonin (PCT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procalcitonin (PCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT). No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procalcitonin (PCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procalcitonin (PCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procalcitonin (PCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procalcitonin (PCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Infection immunity;Hormone metabolism;

References Citing This Product: Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis

Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice

Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia

Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children

The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis

Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis

Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis

The use and value of procalcitonin in solid organ transplantation

Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay

Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension

Risk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy.