The PCR Enhancing Reagent is supplied in 10 x conc. Simply add the reagent in PCR reactions in the 1x-3x final concentration. We recommend that for each target, a series of reactions are set up to titrate the concentration of the PCR Enhancing Reagent in 1x, 2x, and 3 x concentrations.
The PCR Enhancing Reagent is also referred to as PCR Enhancer.
The effective melting temperature of primers in a reaction containing this reagent is reduced. Therefore, a decrease in annealing temperature of PCR is often required.