PCNA antibody , Cat. # FNab06217

SKU: FNab06217

Size:100ug

Purification:Protein A+G purification

Form:liquid

Purity:?95% as determined by SDS-PAGE

Host:Mouse

Clonality:monoclonal

Clone ID:8F9

Isotype:IgG1

Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)

Background:Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Immunogen:proliferating cell nuclear antigen

Synonyms:Proliferating cell nuclear antigen, Cyclin

Observed MW:38 kDa

Uniprot ID:P12004

Reactivity:Human, Mouse, Rat,rabbit

Tested Application:ELISA, WB, IHC, FC, IP

Recommended dilution:WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500

Gene ID:5111

Research Area:Epigenetics, Metabolism, Immunology, Cancer, Cell Division and Proliferation