p53RFP rabbit pAb

SKU: ES3129

Size: 100μL

Source:Rabbit

Applications:WB;ELISA

Reactivity:Human;Mouse

Dilution:Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Immunogen:Synthesized peptide derived from the Internal region of human p53RFP.

Storage_stability:-20°C/1 year

Clonality:Polyclonal

Isotype:IgG

Concentration:1 mg/ml

Observed_band(KD):35kD

Human_gene_id:255488

Human_swiss_prot_no:Q7Z419

Subcellular_location:Mitochondrion membrane ; Single-pass membrane protein . Cytoplasm . Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.

Other_name:RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein

Background:caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymus.,