Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

SKU: EK0527

Size: 96 wells/kit, with removable strips.

Storage: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Form:

Reactivity: Mouse

Applications: ELISA

Application Details:

Gene Name: TNF

Specificity: Natural and recombinant Mouse TNF alpha

Background: Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Immunogen: Expression system for standard: E.coli; Immunogen sequence: L80-L235

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Uniprot ID: P06804

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Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

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Gene ID: 21926

Protein Name: Tumor necrosis factor

Gene Full Name: tumor necrosis factor

Synonyms: Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-a; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor; tnfa; tnfα