Mouse SP-A1(Pulmonarysurfactant-associated protein A1) ELISA Kit

SKU: ELK9615

Size:96T

Reactivity:Mouse

Alternative Names:MGC133365; PSAP; PSPA; SFTP1; SFTPA1

Assay Type:Sandwich

Sensitivity:0.19 ng/mL

Standard:20 ng/mL

Detection range:0.31-20 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Signal Transduction

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SP-A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SP-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SP-A1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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