Mouse Phosphatidylcholine-sterol Acyltransferase, LCAT ELISA Kit

SKU: E2340Mo

Size:96 Tests-1 Kit

Background:Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed: 19065001). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels (PubMed: 19065001). Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins (PubMed: 19065001). Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed: 19065001). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed: 8820107). Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed: 10393212). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF (By similarity). Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters (By similarity).

Research_area:Cardiovascular

Applications:ELISA

Target_protein:LCAT

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.11ng/mL

Detection range:0.2-60ng/mL

Sample type:Serum, plasma or other biological fluids

Full product name:Phosphatidylcholine-Sterol Acyltransferase

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P16301

MASS(Da):49,747

GeneID:16816

Synonyms:1-alkyl-2-acetylglycerophosphocholine esterase;LCAT;Lecithin-cholesterol acyltransferase;PAF acetylhydrolase;Phosphatidylcholine-sterol acyltransferase;Phospholipid-cholesterol acyltransferase;Platelet-activating factor acetylhydrolase

Gene names:LCAT

Standard solution:64ng/mL