Mouse F5(Coagulation Factor V) ELISA Kit

SKU: ELK2927

Size:96T

Reactivity:Mouse

Alternative Names:FV; FVL; PCCF; Proaccelerin; Labile Factor; Factor V Leiden; Accelerator Globulin; Activated protein C cofactor; Proaccelerin, labile factor

Assay Type:Sandwich

Sensitivity:46 pg/mL

Standard:8000 pg/mL

Detection range:125-8000 pg/mL

Sample type:plasma

Assay length:3.5h

Research Area:Enzyme & Kinase;Metabolic pathway;Hematology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F5 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O88783