Mouse DNMT3A(DNA Methyltransferase 3A) ELISA Kit

SKU: ELK9384

Size:96T

Reactivity:Mouse

Alternative Names:DNMT3A2; M.HsaIIIA; DNA methyltransferase HsaIIIA

Assay Type:Sandwich

Sensitivity:0.062 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DNMT3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DNMT3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DNMT3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DNMT3A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O88508