Mouse D-LDH(D-LactateDehydrogenase) ELISA Kit

SKU: ELK9609

Size:96T

Reactivity:Mouse

Alternative Names:DLD; LDHD; Probable D-lactate dehydrogenase;mitochondrial;

Assay Type:Sandwich

Sensitivity:2.74 mIU/mL

Standard:300 mIU/mL

Detection range:4.69-300 mIU/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Signal Transduction;Cancer;Kits/ Lysates/ Other;Metabolism

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse D-LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse D-LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse D-LDH in the samples is then determined by comparing the OD of the samples to the standard curve.

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