Mouse ALCAM(Activated Leukocyte Cell Adhesion Molecule) ELISA Kit

SKU: ELK11224

Size:96T

Reactivity:Mouse

Alternative Names:CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule

Assay Type:Sandwich

Sensitivity:2.17 ng/mL

Standard:500 ng/mL

Detection range:7.81-500 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:CD & Adhesion molecule;Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ALCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ALCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ALCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ALCAM in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q61490