Kim1 ELISA kit (Human)

SKU: SEA785Hu

Size: 96Tests

# of Times Cited in literature: 22

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: Kim1

Target Full Name: Kidney Injury Molecule 1

Alternative Names: HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

Target Species: Human

Uniprot: Q96D42

Gene ID: 26762

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human Kim1 / Kidney Injury Molecule 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 78-5,000pg/mL

Sensitivity: 32pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Urine, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1). No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney Injury Molecule 1 (Kim1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kidney Injury Molecule 1 (Kim1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Infection immunity;Kidney biomarker;

References Citing This Product: Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication

KIM-1 and NGAL: new markers of obstructive nephropathy

Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight Infants

Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rate

Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer Treatment

Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury.

Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant

中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18