GeneBio Systems

IL6 ELISA kit (Human)

Name: IL6 ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEA079Hu
Price: 1029.00 CAD
Availability: InStock

SKU:SEA079Hu

$1,029.00 CAD

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Quantity

Size: 96Tests

# of Times Cited in literature: 180

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: IL6

Target Full Name: Interleukin 6

Alternative Names: MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor

Target Species: Human

Uniprot: P05231

Gene ID: 3569

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human IL6 / Interleukin 6

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 7.8-500pg/mL

Sensitivity: 2.9pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6). No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;

References Citing This Product: Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia

Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa

Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages

The association of interleukin-16 polymorphisms with IL-16 serum levels and risk of colorectal and gastric cancer

Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance

The change of serum level of pigment epithelium-derived factor in coronary heart disease

Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing

Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity

Inflammatory markers and haptoglobin polymorphism in Saudi with non-insulin-dependent diabetes mellitus.

Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats.

Immunogenic alteration in laparoscopic common bile duct exploration.